SM2525 Chemically modified siRNA information

Field Sense Antisense
siRNA chemical modification 2-O-Methyl 2-O-Methyl
siRNA modification types 1 1
Overall number of modifications 11 9
Position of modifications 12,13,14,15,16,17,18,19,20,21 12,13,14,15,16,17,18,19,20
Modification on sugar or base or phosphate Sugar Sugar
SMILES
(Click to view structure & nomenclature)
COC1C(O)OC(CO)C1O



COC1C(O)OC(CO)C1O



siRNA Sequence CUCCUUUUGUUUCUGCUAACG CGUUAGCAGAAACAAAGGAG
siRNA length base-pair 21 20
siRNA name in paper PTEN V8 PTEN V8
Biological activity Low mRNA target inhibition efficacy
Experiment used to check activity Real-time PCR
Melting temperature (oC) NA
Target gene Human PTEN gene
siRNA concentration 1 to 40 nM
Cell or Organism used HeLa cells
Transfection method Oligofectamine (Invitrogen)
Duration after transfection 48 Hours
Article title Structural variations and stabilising modifications of synthetic siRNAs in mammalian cells
Reference12771196