SM2556 Chemically modified siRNA information

Field Sense Antisense
siRNA chemical modification 0 2-Deoxy-2-Fluoro
siRNA modification types 0 1
Overall number of modifications 0 7
Position of modifications 0 1,9,10,12,15,16,19
Modification on sugar or base or phosphate 0 Sugar
SMILES
(Click to view structure & nomenclature)
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OCC1OCC(F)C1O



siRNA Sequence UGGCCAAUGCCAAGGAGAUUU AUCUCCUUGGCAUUGGCCAUU
siRNA length base-pair 21 21
siRNA name in paper A2S1 A2S1
Biological activity 60 percent target mRNA inhibition
Experiment used to check activity Real-time PCR
Melting temperature (oC) 63.3
Target gene Human TRACP 5a and 5b gene
siRNA concentration 1 nM
Cell or Organism used CHO cells
Transfection method Mirus Trans IT TKO (MirusBio, USA)
Duration after transfection 49 Hours
Article title RNA interference tolerates 2'-fluoro modifications at the Argonaute2 cleavage site
Reference17511001