SM2570 Chemically modified siRNA information

Field Sense Antisense
siRNA chemical modification 2-Deoxy-2-Fluoro 2-Deoxy-2-Fluoro
siRNA modification types 1 1
Overall number of modifications 12 14
Position of modifications 2,3,6,7,9,12,13,14,15,16,17,18 2,3,4,5,6,7,8,11,13,14,17,18,20,21
Modification on sugar or base or phosphate Sugar Sugar
SMILES
(Click to view structure & nomenclature)
OCC1OCC(F)C1O



OCC1OCC(F)C1O



siRNA Sequence UGGCCAAUGCCAAGGAGAUUU AUCUCCUUGGCAUUGGCCAUU
siRNA length base-pair 21 21
siRNA name in paper A3S2 A3S2
Biological activity 68 percent target mRNA inhibition
Experiment used to check activity Real-time PCR
Melting temperature (oC) 77.3
Target gene Human TRACP 5a and 5b gene
siRNA concentration 5 nM
Cell or Organism used CHO cells
Transfection method Mirus Trans IT TKO (MirusBio, USA)
Duration after transfection 77 Hours
Article title RNA interference tolerates 2'-fluoro modifications at the Argonaute2 cleavage site
Reference17511001