| Field | Sense | Antisense |
| siRNA chemical modification | Locked nucleic acid | Locked nucleic acid |
| siRNA modification types | 1 | 1 |
| Overall number of modifications | 3 | 2 |
| Position of modifications | 1,20,21 | 20,21 |
| Modification on sugar or base or phosphate | Sugar | Sugar |
| SMILES (Click to view structure & nomenclature) |
OCC12COC(CO1)C2O |
OCC12COC(CO1)C2O |
| siRNA Sequence | GCUGACCCUGAAGUUCAUCTT | GAUGAACUUCAGGGUCAGCTT |
| siRNA length base-pair | 21 | 21 |
| siRNA name in paper | END MODIFIED siGFP | END MODIFIED siGFP |
| Biological activity | 61 percent target mRNA inhibition | |
| Experiment used to check activity | mRNA expression profiling (HG-U133 Plus 2 GeneChips) | |
| Melting temperature (oC) | NA | |
| Target gene | EGFP gene | |
| siRNA concentration | 0.25 mg/kg/d | |
| Cell or Organism used | MiaPaca II cells | |
| Transfection method | Lipofectamine 2000 | |
| Duration after transfection | 7 - 96 Hours | |
| Article title | Evaluation of locked nucleic acid-modified small interfering RNA in vitro and in vivo |
|
| Reference | 17363479 | |