SM2597 Chemically modified siRNA information

Field Sense Antisense
siRNA chemical modification 0 Difluorotoluyl nucleotide
siRNA modification types 0 1
Overall number of modifications 0 1
Position of modifications 0 10
Modification on sugar or base or phosphate 0 Base
SMILES
(Click to view structure & nomenclature)
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CC1=CC(C2OC(CO)C(O)C2O)=C(F)C=C1F



siRNA Sequence CUUACGCUGAGUACUUCGATT UCGAAGUACUCAGCGUAAGTT
siRNA length base-pair 21 21
siRNA name in paper AL-1 AL-10  
Biological activity 57 percent target mRNA inhibition
Experiment used to check activity Dual luciferase reporter assay
Melting temperature (oC) NA
Target gene Luciferase gene
siRNA concentration 10 nM
Cell or Organism used HeLa cells
Transfection method Lipofectamine 2000
Duration after transfection 24 Hours
Article title Crystal structure, stability and in vitro RNAi activity of oligoribonucleotides containing the ribo-difluorotoluyl nucleotide: insights into substrate requirements by the human RISC Ago2 enzyme
Reference17881374