| Field | Sense | Antisense |
| siRNA chemical modification | 2-O-Guanidinoethyl-ribose | 2-O-Aminoethyl-ribose |
| siRNA modification types | 1 | 1 |
| Overall number of modifications | 1 | 2 |
| Position of modifications | 5 | 20,21 |
| Modification on sugar or base or phosphate | Sugar | Sugar |
| SMILES (Click to view structure & nomenclature) |
NC(N)=NCCOC1COC(CO)C1O |
NCCOC1COC(CO)C1O |
| siRNA Sequence | GACGUAAACGGCCACAAGUUC | ACUUGUGGCCGUUUACGUCGCU |
| siRNA length base-pair | 21 | 22 |
| siRNA name in paper | DO0004 | JE1001 |
| Biological activity | 70 percent target mRNA inhibition | |
| Experiment used to check activity | Northern Blot | |
| Melting temperature (oC) | NA | |
| Target gene | EGFP gene | |
| siRNA concentration | 10 nM | |
| Cell or Organism used | H1299 cells | |
| Transfection method | TransIT- TKO | |
| Duration after transfection | 24 Hours | |
| Article title | Synthesis of 2'-O-modified adenosine building blocks and application for RNA interference |
|
| Reference | 17904849 | |