| Field | Sense | Antisense |
| siRNA chemical modification | 2-Fluoro* Phosphorothioate | 2-Fluoro* Phosphorothioate |
| siRNA modification types | 2 | 2 |
| Overall number of modifications | 11 | 11 |
| Position of modifications | 4,6,19,20 * 1,2,3,17,18,19,20 | 2,3,4,15,17,18 * 16,17,18,19,20 |
| Modification on sugar or base or phosphate | Sugar; Phosphate | Sugar; Phosphate |
| SMILES (Click to view structure & nomenclature) |
OCC1OCC(F)C1O OCC1OCC(O)C1OP(O)(S)=O |
OCC1OCC(F)C1O OCC1OCC(O)C1OP(O)(S)=O |
| siRNA Sequence | CGAUGUGUCUAUUGAAGAUUC | AUCUUCAAUAGACACAUCGGC |
| siRNA length base-pair | 21 | 21 |
| siRNA name in paper | R1 | R1 |
| Biological activity | 15 percent target mRNA inhibition | |
| Experiment used to check activity | Dual luciferase reporter assay | |
| Melting temperature (oC) | NA | |
| Target gene | Human SOD1 gene | |
| siRNA concentration | 0.002 nM | |
| Cell or Organism used | HEK293 cells and Transgenic mice FVB/NJ background (SOD1-G93A) | |
| Transfection method | Lipofectamine 2000 | |
| Duration after transfection | 24 Hours | |
| Article title | Therapeutic gene silencing delivered by a chemically modified small interfering RNA against mutant SOD1 slows amyotrophic lateral sclerosis progression |
|
| Reference | 18367449 | |