Field | Sense | Antisense |
siRNA chemical modification | 2-Fluoro* Phosphorothioate | 2-Fluoro* Phosphorothioate |
siRNA modification types | 2 | 2 |
Overall number of modifications | 7 | 6 |
Position of modifications | 6,19,20 * 1,2,19,20 | 2,17,18 * 1,19,20 |
Modification on sugar or base or phosphate | Sugar; Phosphate | Sugar; Phosphate |
SMILES (Click to view structure & nomenclature) |
OCC1OCC(F)C1O OCC1OCC(O)C1OP(O)(S)=O |
OCC1OCC(F)C1O OCC1OCC(O)C1OP(O)(S)=O |
siRNA Sequence | CGAAGUUUGGAUUCAUCAUUC | AUGAUGAAUCCAAACUUCGGC |
siRNA length base-pair | 21 | 21 |
siRNA name in paper | Mm | Mm |
Biological activity | 8 percent target mRNA inhibition | |
Experiment used to check activity | Dual luciferase reporter assay | |
Melting temperature (oC) | NA | |
Target gene | Human SOD1 gene | |
siRNA concentration | 20 nM | |
Cell or Organism used | HEK293 cells and Transgenic mice FVB/NJ background (SOD1-G93A) | |
Transfection method | Lipofectamine 2000 | |
Duration after transfection | 24 Hours | |
Article title | Therapeutic gene silencing delivered by a chemically modified small interfering RNA against mutant SOD1 slows amyotrophic lateral sclerosis progression |
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Reference | 18367449 |