SM2649 Chemically modified siRNA information

Field Sense Antisense
siRNA chemical modification 0 Morpholinouridine
siRNA modification types 0 1
Overall number of modifications 0 1
Position of modifications 0 7
Modification on sugar or base or phosphate 0 Sugar
SMILES
(Click to view structure & nomenclature)
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OCC1CN(CC(O1)N1C=CC(=O)NC1=O)OP(O)([O-])=O



siRNA Sequence CUUACGCUGAGUACUUCGATT UCGAAGUACUCAGCGUAAGTT
siRNA length base-pair 21 21
siRNA name in paper 12 12
Biological activity 67 percent target mRNA inhibition
Experiment used to check activity Dual luciferase reporter assay
Melting temperature (oC) NA
Target gene Luciferase gene
siRNA concentration 25 nM
Cell or Organism used HeLa cells
Transfection method Lipofectamine 2000
Duration after transfection 24 Hours
Article title RNA interference in mammalian cells by siRNAs modified with morpholino nucleoside analogues
Reference19233658