Field | Sense | Antisense |
siRNA chemical modification | Peptide nucleic acids | 0 |
siRNA modification types | 1 | 0 |
Overall number of modifications | 1 | 0 |
Position of modifications | 21 | 0 |
Modification on sugar or base or phosphate | Phosphate | 0 |
SMILES (Click to view structure & nomenclature) |
- |
CNC(=O)CN(C)C(C)=O |
siRNA Sequence | UCGAAGUAUUCCGCGUACGTT | CGUACGCGGAAUACUUCGATT |
siRNA length base-pair | 21 | 21 |
siRNA name in paper | B | B |
Biological activity | 80 percent target mRNA inhibition | |
Experiment used to check activity | Dual luciferase reporter assay | |
Melting temperature (oC) | NA | |
Target gene | Luciferase gene | |
siRNA concentration | 10 nM | |
Cell or Organism used | HeLa cells | |
Transfection method | Cationic liposomes | |
Duration after transfection | 48 Hours | |
Article title | RNA interference in mammalia cells by RNA-3'-PNA chimeras |
|
Reference | 19325750 |