SM2705 Chemically modified siRNA information

Field Sense Antisense
siRNA chemical modification UnLocked nucleic acid 0
siRNA modification types 1 0
Overall number of modifications 1 0
Position of modifications 20 0
Modification on sugar or base or phosphate Sugar 0
SMILES
(Click to view structure & nomenclature)
OCCOC(CO)CO



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siRNA Sequence UUCUCCGAACGUGUCACGUUUU ACGUGACACGUUCGGAGAAUU
siRNA length base-pair 22 21
siRNA name in paper mod 18 mod 18
Biological activity 2.75 (log-10 CVB-3 titre pfu/ml)
Experiment used to check activity Viral titre
Melting temperature (oC) NA
Target gene RNA dependent RNA polymerase (RdRP) gene of CVB-3
siRNA concentration 10 nM
Cell or Organism used Vero and COS7 cells
Transfection method CVB-3 at a multiplicity of infection (m.o.i.) of 0.1
Duration after transfection 19 Hours
Article title Application of small interfering RNAs modified by unlocked nucleic acid (UNA) to inhibit the heart-pathogenic coxsackievirus B3
Reference20005874