SM2796 Chemically modified siRNA information

Field Sense Antisense
siRNA chemical modification 2-Deoxynebularine 0
siRNA modification types 1 0
Overall number of modifications 1 0
Position of modifications 12 0
Modification on sugar or base or phosphate Base 0
SMILES
(Click to view structure & nomenclature)
OCC1OC(CC1O)N1C=NC2=CN=CN=C12



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siRNA Sequence CUUACGCUGAGUACUUCGATT TCGAAGUACUCAGCGUAAGTT
siRNA length base-pair 21 21
siRNA name in paper U12 -> dNeb U12 -> dNeb
Biological activity NA
Experiment used to check activity Firefly luciferase assay and chemoluminescent assay
Melting temperature (oC) 64.2
Target gene Luciferase gene
siRNA concentration 0.002 to 8.0 nM
Cell or Organism used HeLa cells
Transfection method Lipofectamine 2000
Duration after transfection 24 Hours
Article title Modulation of thermal stability can enhance the potency of siRNA
Reference20610434