Field | Sense | Antisense |
siRNA chemical modification | 2-O-Methyl* 2,4-Difluorotoluene* Phosphorothioate | 2-O-Methyl* Phosphorothioate |
siRNA modification types | 3 | 2 |
Overall number of modifications | 10 | 3 |
Position of modifications | 4,6,10,11,13,14,15,18 * 9 *20 | 8,13 * 20 |
Modification on sugar or base or phosphate | Sugar; Base; Phosphate | Sugar; Phosphate |
SMILES (Click to view structure & nomenclature) |
COC1C(O)OC(CO)C1O CC1=CC(CC2OC(CO)C(O)C2O)=C(F)C=C1F OCC1OCC(O)C1OP(O)(S)=O |
COC1C(O)OC(CO)C1O OCC1OCC(O)C1OP(O)(S)=O |
siRNA Sequence | GAACGUGAAUUGCUCAACATT | UGUUGAGCAAUUCACGUUCTT |
siRNA length base-pair | 21 | 21 |
siRNA name in paper | A9 -> F (siRNA-3) | A9 -> F (siRNA-3) |
Biological activity | IC-50 = 0.221 nM for target mRNA | |
Experiment used to check activity | Firefly luciferase assay and chemoluminescent assay | |
Melting temperature (oC) | NA | |
Target gene | Luciferase gene | |
siRNA concentration | 0.002 to 8.0 nM | |
Cell or Organism used | HeLa cells | |
Transfection method | Lipofectamine 2000 | |
Duration after transfection | 24 Hours | |
Article title | Modulation of thermal stability can enhance the potency of siRNA |
|
Reference | 20610434 |