| Field | Sense | Antisense |
| siRNA chemical modification | 2,4-difluorotoluene ribonucleotide | 0 |
| siRNA modification types | 1 | 0 |
| Overall number of modifications | 1 | 0 |
| Position of modifications | 12 | 0 |
| Modification on sugar or base or phosphate | Base | 0 |
| SMILES (Click to view structure & nomenclature) |
CC1=CC(CC2OC(CO)C(O)C2O)=C(F)C=C1F |
- |
| siRNA Sequence | AAGUAAGGACCAGAGACAATT | UUGUCUCUGGUCCUUACUUTT |
| siRNA length base-pair | 21 | 21 |
| siRNA name in paper | A12 -> F (siRNA-4) | A12 -> F (siRNA-4) |
| Biological activity | IC-50 = 0.027 nM for target mRNA | |
| Experiment used to check activity | Firefly luciferase assay and chemoluminescent assay | |
| Melting temperature (oC) | 63.9 | |
| Target gene | PTEN | |
| siRNA concentration | 0.002 to 8.0 nM | |
| Cell or Organism used | HeLa cells | |
| Transfection method | Lipofectamine 2011 | |
| Duration after transfection | 35 Hours | |
| Article title | Modulation of thermal stability can enhance the potency of siRNA |
|
| Reference | 20610434 | |