SM2857 Chemically modified siRNA information

Field Sense Antisense
siRNA chemical modification 0 Hypoxanthine
siRNA modification types 0 1
Overall number of modifications 0 1
Position of modifications 0 9
Modification on sugar or base or phosphate 0 Base
SMILES
(Click to view structure & nomenclature)
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O=C1N=CNC2=C1NC=N2



siRNA Sequence CAAGUGUCAUCACACUGAATT UUCAGUGUGAUGACACUUGTT
siRNA length base-pair 21 21
siRNA name in paper A9 A9
Biological activity 54 percent target mRNA inhibition
Experiment used to check activity Real-time PCR
Melting temperature (oC) NA
Target gene Apolipoprotein B gene
siRNA concentration 10 nM
Cell or Organism used Hepa1-6 cells
Transfection method Lipofectamine RNAiMAX (Invitrogen)
Duration after transfection 24 Hours
Article title Nucleoside optimization for RNAi: a high-throughput platform
Reference21942264