| Field | Sense | Antisense |
| siRNA chemical modification | 0 | Hypoxanthine* 2-O-Benzyl |
| siRNA modification types | 0 | 2 |
| Overall number of modifications | 0 | 2 |
| Position of modifications | 0 | 6 * 6 |
| Modification on sugar or base or phosphate | 0 | Base; Sugar |
| SMILES (Click to view structure & nomenclature) |
- |
O=C1N=CNC2=C1NC=N2 OCC1OCC(OC2=CC=CC=C2)C1O |
| siRNA Sequence | CAAGUGUCAUCACACUGAATT | UUCAGUGUGAUGACACUUGTT |
| siRNA length base-pair | 21 | 21 |
| siRNA name in paper | B6 | B6 |
| Biological activity | 82 percent target mRNA inhibition | |
| Experiment used to check activity | Real-time PCR | |
| Melting temperature (oC) | NA | |
| Target gene | Apolipoprotein B gene | |
| siRNA concentration | 10 nM | |
| Cell or Organism used | Hepa1-6 cells | |
| Transfection method | Lipofectamine RNAiMAX (Invitrogen) | |
| Duration after transfection | 24 Hours | |
| Article title | Nucleoside optimization for RNAi: a high-throughput platform |
|
| Reference | 21942264 | |