Field | Sense | Antisense |
siRNA chemical modification | 0 | 2-O-Benzyl |
siRNA modification types | 0 | 1 |
Overall number of modifications | 0 | 1 |
Position of modifications | 0 | 19 |
Modification on sugar or base or phosphate | 0 | Sugar |
SMILES (Click to view structure & nomenclature) |
- |
OCC1OCC(OC2=CC=CC=C2)C1O |
siRNA Sequence | CAAGUGUCAUCACACUGAATT | UUCAGUGUGAUGACACUUGTT |
siRNA length base-pair | 21 | 21 |
siRNA name in paper | C19 | C19 |
Biological activity | 80 percent target mRNA inhibition | |
Experiment used to check activity | Real-time PCR | |
Melting temperature (oC) | NA | |
Target gene | Apolipoprotein B gene | |
siRNA concentration | 10 nM | |
Cell or Organism used | Hepa1-6 cells | |
Transfection method | Lipofectamine RNAiMAX (Invitrogen) | |
Duration after transfection | 24 Hours | |
Article title | Nucleoside optimization for RNAi: a high-throughput platform |
|
Reference | 21942264 |