SM2921 Chemically modified siRNA information

Field Sense Antisense
siRNA chemical modification UnLocked nucleic acid Unlocked nucleic acid * Locked nucleic acid
siRNA modification types 1 2
Overall number of modifications 1 3
Position of modifications 21 6 * 20,21
Modification on sugar or base or phosphate Sugar Sugar
SMILES
(Click to view structure & nomenclature)
OCCOC(CO)CO



OCCOC(CO)CO



siRNA Sequence GACGUAAACGGCCACAAGUUUU ACUUGUGGCCGUUUACGUCGCU
siRNA length base-pair 22 22
siRNA name in paper UNA modified 2 UNA modified 2
Biological activity High target mRNA inhibition efficacy
Experiment used to check activity Northern Blot
Melting temperature (oC) NA
Target gene EGFP gene
siRNA concentration 5 nM
Cell or Organism used MiaPaca II cells
Transfection method Lipofectamin 2027
Duration after transfection 24 Hours
Article title In vivo efficacy and off-target effects of locked nucleic acid (LNA) and unlocked nucleic acid (UNA) modified siRNA and small internally segmented interfering RNA (sisiRNA) in mice bearing human tumor xenografts
Reference21687525