Field | Sense | Antisense |
siRNA chemical modification | Phosphorothioate | Phosphorothioate |
siRNA modification types | 1 | 1 |
Overall number of modifications | 6 | 6 |
Position of modifications | 2,3,4,25,26,27 | 2,3,4,25,26,27 |
Modification on sugar or base or phosphate | Phosphate | Phosphate |
SMILES (Click to view structure & nomenclature) |
OCC1OCC(O)C1OP(O)(S)=O |
OCC1OCC(O)C1OP(O)(S)=O |
siRNA Sequence | AAGCUGACCCUGAAGUUCAUCUGCACC | GGUGCAGAUGAACUUCAGGGUCAGCUU |
siRNA length base-pair | 27 | 27 |
siRNA name in paper | PS6 | PS6 |
Biological activity | 93 percent target mRNA inhibition | |
Experiment used to check activity | GFP signal 10 minute UV exposure | |
Melting temperature (oC) | NA | |
Target gene | GFP gene | |
siRNA concentration | 1.56 nM | |
Cell or Organism used | HeLa cells | |
Transfection method | Lipofectamine (Invitrogen) | |
Duration after transfection | 42 Hours | |
Article title | Enhanced light-activated RNA interference using phosphorothioate-based dsRNA precursors of siRNA |
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Reference | 21739319 |