SM2951 Chemically modified siRNA information

Field Sense Antisense
siRNA chemical modification Phosphorothioate* Dimethoxy-Nitrophenyl Ethyl group Phosphorothioate* Dimethoxy-Nitrophenyl Ethyl group
siRNA modification types 2 2
Overall number of modifications 8 8
Position of modifications 2,3,4,25,26,27 * 1,27 2,3,4,25,26,27 * 1,27
Modification on sugar or base or phosphate Phosphate Phosphate
SMILES
(Click to view structure & nomenclature) 		OCC1OCC(O)C1OP(O)(S)=O
COC1=C(OC)C=C(C(C)=C1)[N+]([O-])=O
OCC1OCC(O)C1OP(O)(S)=O
COC1=C(OC)C=C(C(C)=C1)[N+]([O-])=O
siRNA Sequence AAGCUGACCCUGAAGUUCAUCUGCACC GGUGCAGAUGAACUUCAGGGUCAGCUU
siRNA length base-pair 27 27
siRNA name in paper PS6-DMNPE PS6-DMNPE
Biological activity 88 percent target mRNA inhibition
Experiment used to check activity GFP signal 10 minute UV exposure
Melting temperature (oC) NA
Target gene GFP gene
siRNA concentration 1.56 nM
Cell or Organism used HeLa cells
Transfection method Lipofectamine (Invitrogen)
Duration after transfection 42 Hours
Article title Enhanced light-activated RNA interference using phosphorothioate-based dsRNA precursors of siRNA
Reference21739319