| Field | Sense | Antisense |
| siRNA chemical modification | 0 | Unlocked nucleic acid |
| siRNA modification types | 0 | 1 |
| Overall number of modifications | 0 | 1 |
| Position of modifications | 0 | 18 |
| Modification on sugar or base or phosphate | 0 | Sugar |
| SMILES (Click to view structure & nomenclature) |
- |
OCCOC(CO)CO |
| siRNA Sequence | GUAUGACCGACUACGCGUATT | UUCAGUGUGAUGACACUUGTT |
| siRNA length base-pair | 21 | 21 |
| siRNA name in paper | UNA18 | UNA18 |
| Biological activity | 91 percent target mRNA inhibition (IC-50 = 1.02 nM) | |
| Experiment used to check activity | Real-time PCR | |
| Melting temperature (oC) | 65 | |
| Target gene | Apolipoprotein B gene | |
| siRNA concentration | 10 nM | |
| Cell or Organism used | Hepa1-6 cells | |
| Transfection method | Lipofectamine RNAiMAX (Invitrogen) | |
| Duration after transfection | 24 Hours | |
| Article title | Analysis of acyclic nucleoside modifications in siRNAs finds sensitivity at position 1 that is restored by 5'-terminal phosphorylation both in vitro and in vivo |
|
| Reference | 19917641 | |