Field | Sense | Antisense |
siRNA chemical modification | 2-Fluoro | 2-Fluoro |
siRNA modification types | 1 | 1 |
Overall number of modifications | 10 | 9 |
Position of modifications | 2,5,7,10,11,12,13,14,15,16 | 1,2,3,11,12,14,16,17,19 |
Modification on sugar or base or phosphate | Sugar | Sugar |
SMILES (Click to view structure & nomenclature) |
OCC1OCC(F)C1O |
OCC1OCC(F)C1O |
siRNA Sequence | GCAGCACGACUUCUUCAAGTT | CUUGAAGAAGUCGUGCUGCTT |
siRNA length base-pair | 21 | 21 |
siRNA name in paper | DS-2-FU, FC | DS-2-FU, FC |
Biological activity | 83 percent target mRNA inhibition | |
Experiment used to check activity | Dual fluorescence assay | |
Melting temperature (oC) | NA | |
Target gene | EGFP gene | |
siRNA concentration | 50 nM | |
Cell or Organism used | HeLa cells | |
Transfection method | Lipofectamine (Invitrogen) | |
Duration after transfection | 4 -144 Hours | |
Article title | siRNA function in RNAi: a chemical modification analysis |
|
Reference | 12923253 |