SM2983 Chemically modified siRNA information

Field Sense Antisense
siRNA chemical modification 2-Fluoro 2-Fluoro* 2-Deoxy
siRNA modification types 1 2
Overall number of modifications 10 19
Position of modifications 2,5,7,10,11,12,13,14,15,16 1,2,3,11,12,14,16,17,19 * 4,5,6,7,8,9,10,13,15,18
Modification on sugar or base or phosphate Sugar Sugar
SMILES
(Click to view structure & nomenclature) 		OCC1OCC(F)C1O
OCC1OCC(F)C1O
OCC1OCCC1O
siRNA Sequence GCAGCACGACUUCUUCAAGTT CUUGAAGAAGUCGUGCUGCTT
siRNA length base-pair 21 21
siRNA name in paper ss-2-FU, FC/AS-2-FU, FC +dA,dG ss-2-FU, FC/AS-2-FU, FC +dA,dG
Biological activity 44 percent target mRNA inhibition
Experiment used to check activity Dual fluorescence assay
Melting temperature (oC) NA
Target gene EGFP gene
siRNA concentration 50 nM
Cell or Organism used HeLa cells
Transfection method Lipofectamine (Invitrogen)
Duration after transfection 4 -144 Hours
Article title siRNA function in RNAi: a chemical modification analysis
Reference12923253