Field | Sense | Antisense |
siRNA chemical modification | Locked nucleic acid | Locked nucleic acid |
siRNA modification types | 1 | 1 |
Overall number of modifications | 2 | 2 |
Position of modifications | 20,21 | 20,21 |
Modification on sugar or base or phosphate | Sugar | Sugar |
SMILES (Click to view structure & nomenclature) |
OCC12COC(CO1)C2O |
OCC12COC(CO1)C2O |
siRNA Sequence | GACGUAAACGGCCACAAGUUCU | ACUUGUGGCCGUUUACGUCGCU |
siRNA length base-pair | 22 | 22 |
siRNA name in paper | W194 | W208 |
Biological activity | 92 percent target mRNA inhibition | |
Experiment used to check activity | Dual luciferase reporter assay | |
Melting temperature (oC) | NA | |
Target gene | EGFP gene | |
siRNA concentration | 10 nM | |
Cell or Organism used | H1299 cells | |
Transfection method | Lipofectamine 2000 | |
Duration after transfection | 48 Hours | |
Article title | In vivo screening of modified siRNAs for non-specific antiviral effect in a small fish model: number and localization in the strands are important |
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Reference | 22287630 |