SM3021 Chemically modified siRNA information

Field Sense Antisense
siRNA chemical modification 0 Phosphorothioate
siRNA modification types 0 1
Overall number of modifications 0 4
Position of modifications 0 4,7,10,19
Modification on sugar or base or phosphate 0 Phosphate
SMILES
(Click to view structure & nomenclature)
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OCC1OCC(O)C1OP(O)(S)=O



siRNA Sequence GUUCACCUUGAUGCCGUUCUU GAACGGCAUCAAGGUGAACUU
siRNA length base-pair 21 21
siRNA name in paper EGFP1 ANSTC EGFP1 ANSTC
Biological activity 58 percent target mRNA inhibition
Experiment used to check activity FACS and cellular RNA analysis
Melting temperature (oC) NA
Target gene EGFP gene
siRNA concentration 25 nM
Cell or Organism used HeLa cells
Transfection method Oligofectamine (Invitrogen)
Duration after transfection 54 Hours
Article title RNA interference using boranophosphate siRNAs: structure-activity relationships
Reference15545637