Field | Sense | Antisense |
siRNA chemical modification | 0 | Boranophosphate |
siRNA modification types | 0 | 1 |
Overall number of modifications | 0 | 4 |
Position of modifications | 0 | 4,7,10,19 |
Modification on sugar or base or phosphate | 0 | Phosphate |
SMILES (Click to view structure & nomenclature) |
- |
BP(O)(=O)OC1C(O)COC1CO |
siRNA Sequence | GUUCACCUUGAUGCCGUUCUU | GAACGGCAUCAAGGUGAACUU |
siRNA length base-pair | 21 | 21 |
siRNA name in paper | EGFP1 ANSBC | EGFP1 ANSBC |
Biological activity | 91 percent target mRNA inhibition | |
Experiment used to check activity | FACS and cellular RNA analysis | |
Melting temperature (oC) | NA | |
Target gene | EGFP gene | |
siRNA concentration | 25 nM | |
Cell or Organism used | HeLa cells | |
Transfection method | Oligofectamine (Invitrogen) | |
Duration after transfection | 54 Hours | |
Article title | RNA interference using boranophosphate siRNAs: structure-activity relationships |
|
Reference | 15545637 |