SM3065 Chemically modified siRNA information

Field Sense Antisense
siRNA chemical modification Locked nucleic acid Locked nucleic acid
siRNA modification types 1 1
Overall number of modifications 3 3
Position of modifications 1,20,21 1,20,21
Modification on sugar or base or phosphate Sugar Sugar
SMILES
(Click to view structure & nomenclature)
OCC12COC(CO1)C2O



OCC12COC(CO1)C2O



siRNA Sequence TGAGAGAAAGCACAGAAAATT TUUUCUGUGCUUUCUCUCATT
siRNA length base-pair 21 21
siRNA name in paper siLNA21 siLNA21
Biological activity 67 percent target mRNA inhibition
Experiment used to check activity Luciferase reporter assay
Melting temperature (oC) NA
Target gene Luciferase gene
siRNA concentration 13 nM
Cell or Organism used HEK293, rat PC12 and Vero cells
Transfection method Lipofectamine 2000
Duration after transfection 24 Hours
Article title Locked nucleic acid (LNA) mediated improvements in siRNA stability and functionality
Reference15653644