Field | Sense | Antisense |
siRNA chemical modification | 0 | 2-Deoxy-2-Fluoroarabinonucleic acid |
siRNA modification types | 0 | 1 |
Overall number of modifications | 0 | 1 |
Position of modifications | 0 | 13 |
Modification on sugar or base or phosphate | 0 | Sugar |
SMILES (Click to view structure & nomenclature) |
- |
C1C(C(C(O1)CO)O)F |
siRNA Sequence | GCUUGAAGUCUUUAAUUAATT | UUAAUUAAAGACTUCAAGCGG |
siRNA length base-pair | 21 | 21 |
siRNA name in paper | F2 | F2 |
Biological activity | IC-50 = 0.31 nM for target mRNA | |
Experiment used to check activity | Luciferase reporter assay | |
Melting temperature (oC) | 60 | |
Target gene | Luciferase gene | |
siRNA concentration | 0.31 nM | |
Cell or Organism used | HeLa X1/5 cells | |
Transfection method | Lipofectamine-plus reagent (Invitrogen) | |
Duration after transfection | 24 Hours | |
Article title | 2'-fluoro-4'-thioarabino-modified oligonucleotides: conformational switches linked to siRNA activity |
|
Reference | 17284457 |