SM3096 Chemically modified siRNA information

Field Sense Antisense
siRNA chemical modification 0 2-Deoxy-2-Fluoro-4-Thioarabinonucleic acid
siRNA modification types 0 1
Overall number of modifications 0 2
Position of modifications 0 1,2
Modification on sugar or base or phosphate 0 Sugar
SMILES
(Click to view structure & nomenclature)
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OCC1SCC(F)C1O



siRNA Sequence GCUUGAAGUCUUUAAUUAATT UUAAUUAAAGACUUCAAGCGG
siRNA length base-pair 21 21
siRNA name in paper T3P T3P
Biological activity IC-50 = 0.16 nM for target mRNA
Experiment used to check activity Luciferase reporter assay
Melting temperature (oC) NA
Target gene Luciferase gene
siRNA concentration 1.16 nM
Cell or Organism used HeLa X1/5 cells
Transfection method Lipofectamine-plus reagent (Invitrogen)
Duration after transfection 24 Hours
Article title 2'-fluoro-4'-thioarabino-modified oligonucleotides: conformational switches linked to siRNA activity
Reference17284457