Field | Sense | Antisense |
siRNA chemical modification | 0 | 2,4-Difluorobenzene |
siRNA modification types | 0 | 1 |
Overall number of modifications | 0 | 1 |
Position of modifications | 0 | 5 |
Modification on sugar or base or phosphate | 0 | Base |
SMILES (Click to view structure & nomenclature) |
- |
OCC1OC(C(O)C1O)C1=C(F)C=C(F)C=C1 |
siRNA Sequence | ACCUGACGUUGUACAAAUUTT | AAUUUGUACAACGUCAGGUTT |
siRNA length base-pair | 21 | 21 |
siRNA name in paper | 5F | 5F |
Biological activity | 26 percent target mRNA inhibition | |
Experiment used to check activity | Dual luciferase reporter assay | |
Melting temperature (oC) | 65 | |
Target gene | Luciferase gene | |
siRNA concentration | 2.1 ng | |
Cell or Organism used | HeLa cells | |
Transfection method | Liposomes | |
Duration after transfection | 22 Hours | |
Article title | Steric effects in RNA interference: probing the influence of nucleobase size and shape |
|
Reference | 18624291 |