SM3144 Chemically modified siRNA information

Field Sense Antisense
siRNA chemical modification 0 2-Fluoro* 2-O-Methyl
siRNA modification types 0 2
Overall number of modifications 0 19
Position of modifications 0 8,9,10,11,13,14,17,18,19,20,23,25,26 * 12,15,16,21,22,24
Modification on sugar or base or phosphate 0 Sugar
SMILES
(Click to view structure & nomenclature)
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OCC1OCC(F)C1O
COC1C(O)OC(CO)C1O


siRNA Sequence GCACCAGAGCCAAUGGAACUUGATG CAUCAAGUUCCAUUGGCUCUGGUGCUU
siRNA length base-pair 25 27
siRNA name in paper STAT1-15 STAT1-15
Biological activity 79 percent target mRNA inhibition
Experiment used to check activity Real-time PCR
Melting temperature (oC) NA
Target gene STAT1 gene
siRNA concentration 10 nM
Cell or Organism used HeLa cells
Transfection method TriFECTin (Integrated DNA Technologies, Coralville, IA)
Duration after transfection 24 Hours
Article title Chemical modification patterns compatible with high potency dicer-substrate small interfering RNAs
Reference18637735