SM3158 Chemically modified siRNA information

Field Sense Antisense
siRNA chemical modification 0 2-Fluoro
siRNA modification types 0 1
Overall number of modifications 0 13
Position of modifications 0 8,9,10,11,13,14,17,18,19,20,23,25,26
Modification on sugar or base or phosphate 0 Sugar
SMILES
(Click to view structure & nomenclature)
-



OCC1OCC(F)C1O



siRNA Sequence GCACCAGAGCCAAUGGAACUUGATG CAUCAAGUUCCAUUGGCUCUGGUGCUU
siRNA length base-pair 25 27
siRNA name in paper STAT1-11 STAT1-11
Biological activity 88 percent target mRNA inhibition
Experiment used to check activity Real-time PCR
Melting temperature (oC) NA
Target gene STAT1 gene
siRNA concentration 1 nM
Cell or Organism used HeLa cells
Transfection method TriFECTin (Integrated DNA Technologies, Coralville, IA)
Duration after transfection 24 Hours
Article title Chemical modification patterns compatible with high potency dicer-substrate small interfering RNAs
Reference18637735