Field | Sense | Antisense |
siRNA chemical modification | 2-O-Methyl | 0 |
siRNA modification types | 1 | 0 |
Overall number of modifications | 9 | 0 |
Position of modifications | 2,4,6,8,10,12,14,16,18 | 0 |
Modification on sugar or base or phosphate | Sugar | 0 |
SMILES (Click to view structure & nomenclature) |
COC1C(O)OC(CO)C1O |
- |
siRNA Sequence | ACCCUGAAGUUCAUCUGCACCACCG | CGGUGGUGCAGAUGAACUUCAGGGUCA |
siRNA length base-pair | 25 | 27 |
siRNA name in paper | EGFP-2 | EGFP-2 |
Biological activity | 31 percent target mRNA inhibition | |
Experiment used to check activity | Real-time PCR | |
Melting temperature (oC) | NA | |
Target gene | EGFP gene | |
siRNA concentration | 0.1 nM | |
Cell or Organism used | HeLa cells | |
Transfection method | TriFECTin (Integrated DNA Technologies, Coralville, IA) | |
Duration after transfection | 24 Hours | |
Article title | Chemical modification patterns compatible with high potency dicer-substrate small interfering RNAs |
|
Reference | 18637735 |