SM3238 Chemically modified siRNA information

Field Sense Antisense
siRNA chemical modification Tricyclodeoxyribonucleic acid 0
siRNA modification types 1 0
Overall number of modifications 6 0
Position of modifications 13,14,15,16,20,21 0
Modification on sugar or base or phosphate Sugar 0
SMILES
(Click to view structure & nomenclature)
OC1CCC2(O)CCOC12



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siRNA Sequence GCAGCACGACUUCTTCAAGTT CUUGAAGAAGUCGUGCUGCTT
siRNA length base-pair 21 21
siRNA name in paper siTCS3' siTCS3'
Biological activity 76 percent target mRNA inhibition
Experiment used to check activity FACS and Real-time PCR
Melting temperature (oC) NA
Target gene EGFP gene
siRNA concentration 20 nM
Cell or Organism used HeLa cells
Transfection method Lipofectamine (Invitrogen)
Duration after transfection 42 Hours
Article title Improving gene silencing of siRNAs via tricyclo-DNA modification
Reference21687522