SM3329 Chemically modified siRNA information

Field Sense Antisense
siRNA chemical modification 2-O-Methyl 0
siRNA modification types 1 0
Overall number of modifications 11 0
Position of modifications 19,21,23,25,27,29,31,33,35,37,39 0
Modification on sugar or base or phosphate Sugar 0
SMILES
(Click to view structure & nomenclature)
COC1C(O)OC(CO)C1O



-



siRNA Sequence UGAGGUUUAGGAUUCGUGCUUGCACGAAUCCUAAACCUCA 0
siRNA length base-pair 40 0
siRNA name in paper SG217 0
Biological activity 88 percent target mRNA inhibition (IC-50 = 0.0065 nM)
Experiment used to check activity Luciferase activity (MicroLumat LB 96P)
Melting temperature (oC) NA
Target gene Luciferase gene
siRNA concentration 20 nM
Cell or Organism used HEK293 cells
Transfection method Lipofectamine 2000
Duration after transfection 48 Hours
Article title Effects of chemical modification on the potency, serum stability, and immunostimulatory properties of short shRNAs
Reference19948766