| Field | Sense | Antisense |
| siRNA chemical modification | 2-O-Methyl | 0 |
| siRNA modification types | 1 | 0 |
| Overall number of modifications | 11 | 0 |
| Position of modifications | 19,21,23,25,27,29,31,33,35,37,39 | 0 |
| Modification on sugar or base or phosphate | Sugar | 0 |
| SMILES (Click to view structure & nomenclature) |
COC1C(O)OC(CO)C1O |
- |
| siRNA Sequence | UGAGGUUUAGGAUUCGUGCUUGCACGAAUCCUAAACCUCA | 0 |
| siRNA length base-pair | 40 | 0 |
| siRNA name in paper | SG217 | 0 |
| Biological activity | 88 percent target mRNA inhibition (IC-50 = 0.0065 nM) | |
| Experiment used to check activity | Luciferase activity (MicroLumat LB 96P) | |
| Melting temperature (oC) | NA | |
| Target gene | Luciferase gene | |
| siRNA concentration | 20 nM | |
| Cell or Organism used | HEK293 cells | |
| Transfection method | Lipofectamine 2000 | |
| Duration after transfection | 48 Hours | |
| Article title | Effects of chemical modification on the potency, serum stability, and immunostimulatory properties of short shRNAs |
|
| Reference | 19948766 | |