Field | Sense | Antisense |
siRNA chemical modification | 0 | 2-Deoxy-2-fluorouridine |
siRNA modification types | 0 | 1 |
Overall number of modifications | 0 | 2 |
Position of modifications | 0 | 3,20 |
Modification on sugar or base or phosphate | 0 | Sugar |
SMILES (Click to view structure & nomenclature) |
- |
OCC1OC(C(F)C1O)N1C=CC(=O)NC1=O |
siRNA Sequence | AGACGAGCUGAGCGAGAAGTT | TTUCUGCUCGACUCGCUCUUC |
siRNA length base-pair | 21 | 21 |
siRNA name in paper | F3 | F3 |
Biological activity | NA | |
Experiment used to check activity | NA | |
Melting temperature (oC) | 80 | |
Target gene | Human Caveolin-1 gene | |
siRNA concentration | 200 nM | |
Cell or Organism used | HeLa cells | |
Transfection method | Oligofectamine (Invitrogen) | |
Duration after transfection | 10-14 Hours | |
Article title | RNA interference in mammalian cells by chemically-modified RNA |
|
Reference | 12834349 |