| Field | Sense | Antisense |
| siRNA chemical modification | Deoxy* 2-Fluoro* Inverted deoxy abasic | Phosphorothioate* Deoxy* 2-Fluoro* 2-O-Methyl |
| siRNA modification types | 2 | 5 |
| Overall number of modifications | 21 | 20 |
| Position of modifications | 1,2,3,12,13,20,21 * 4,5,6,7,8,9,10,11,14,15,16,17,18,19* 1,21 | 21 * 1,2,3 * 7,8,17,18,19 * 9,10,11,12,13,14,15,16 * 1,21 |
| Modification on sugar or base or phosphate | Sugar | Phosphate; Sugar; Sugar; Sugar |
| SMILES (Click to view structure & nomenclature) |
OCC1OCCC1O OCC1OCC(F)C1O OCC1OCCC1OP(O)([O-])=O |
OCC1OCC(O)C1OP(O)(S)=O OCC1OCCC1O OCC1OCC(F)C1O COC1C(O)OC(CO)C1O |
| siRNA Sequence | GGACUUCUCUCAAUUUUCUTT | AGAAAAUUGAGAGAAGUCCTT |
| siRNA length base-pair | 21 | 21 |
| siRNA name in paper | 263 | 263 |
| Biological activity | NA | |
| Experiment used to check activity | ELISA | |
| Melting temperature (oC) | NA | |
| Target gene | 0 | |
| siRNA concentration | 0.5 to 25 nM | |
| Cell or Organism used | Hep G2 cells | |
| Transfection method | NA | |
| Duration after transfection | 72 Hours | |
| Article title | Potent and persistent in vivo anti-HBV activity of chemically modified siRNAs |
|
| Reference | 16041363 | |