| Field | Sense | Antisense |
| siRNA chemical modification | Phosphorothioate | Phosphorothioate* 2-O-Methyl |
| siRNA modification types | 1 | 2 |
| Overall number of modifications | 1 | 4 |
| Position of modifications | 21 | 21,22 * 21,22 |
| Modification on sugar or base or phosphate | Phosphate | Phosphate; Sugar |
| SMILES (Click to view structure & nomenclature) |
OCC1OCC(O)C1OP(O)(S)=O |
OCC1OCC(O)C1OP(O)(S)=O COC1C(O)OC(CO)C1O |
| siRNA Sequence | GAACUGUGUGUGAGAGGUCCU | AGGACCUCUCACACACAGUUCGC |
| siRNA length base-pair | 21 | 23 |
| siRNA name in paper | siLuc | siLuc |
| Biological activity | 80 percent target mRNA inhibition | |
| Experiment used to check activity | Luciferase reporter assay | |
| Melting temperature (oC) | NA | |
| Target gene | Luciferase gene | |
| siRNA concentration | 25 to 500 nM | |
| Cell or Organism used | COS-7 cells | |
| Transfection method | NA | |
| Duration after transfection | NA | |
| Article title | Development of novel nucleic acid-loaded Bubble liposomes using cholesterol-conjugated siRNA |
|
| Reference | 21663557 | |