| Field | Sense | Antisense |
| siRNA chemical modification | 2-O-Lysylaminohexyl | 2-O-Lysylaminohexyl |
| siRNA modification types | 1 | 1 |
| Overall number of modifications | 2 | 2 |
| Position of modifications | 6,18 | 7,14 |
| Modification on sugar or base or phosphate | Sugar | Sugar |
| SMILES (Click to view structure & nomenclature) |
CCC1OCC(OCCCCCCNC(=O)C(CCCCNC(=O)C(F)(F)F)NC(=O)C(F)(F)F)C1O |
CCC1OCC(OCCCCCCNC(=O)C(CCCCNC(=O)C(F)(F)F)NC(=O)C(F)(F)F)C1O |
| siRNA Sequence | TTAUCUCCAUGCACGACUCCG | UAGAGGUACGUGCUGAGGCTT |
| siRNA length base-pair | 21 | 21 |
| siRNA name in paper | 27 | 27 |
| Biological activity | 37 percent target mRNA inhibition | |
| Experiment used to check activity | HPLC analysis | |
| Melting temperature (oC) | NA | |
| Target gene | ICAM-1 gene | |
| siRNA concentration | 25 and 50 nM | |
| Cell or Organism used | ECV304 | |
| Transfection method | Lipofectamine 2000 | |
| Duration after transfection | 24 Hours | |
| Article title | 2'-O-Lysylaminohexyl oligonucleotides: modifications for antisense and siRNA |
|
| Reference | 17979170 | |