SM3387 Chemically modified siRNA information

Field Sense Antisense
siRNA chemical modification 2-Deoxy-2-Fluoro* 2-O-Methyl 2-Deoxy-2-Fluoro* 2-O-Methyl
siRNA modification types 2 2
Overall number of modifications 21 21
Position of modifications 1,3,5,7,9,11,13,15,17,19,21 * 2,4,6,8,10,12,14,16,18,20 2,4,6,8,10,12,14,16,18,20 * 1,3,5,7,9,11,13,15,17,19,21
Modification on sugar or base or phosphate Sugar Sugar
SMILES
(Click to view structure & nomenclature)
OCC1OCC(F)C1O
COC1C(O)OC(CO)C1O


OCC1OCC(F)C1O
COC1C(O)OC(CO)C1O


siRNA Sequence GGGUAAAUACAUUCUUCAUUU AUGAAGAAUGUAUUUACCCUU
siRNA length base-pair 21 21
siRNA name in paper ISIS 401156 ISIS 401157
Biological activity NA
Experiment used to check activity NA
Melting temperature (oC) 20
Target gene 0
siRNA concentration 2.0 mM
Cell or Organism used HeLa cells
Transfection method NA
Duration after transfection NA
Article title Solution-state structure of a fully alternately 2'-F/2'-OMe modified 42-nt dimeric siRNA construct
Reference20624819