SM3392 Chemically modified siRNA information

Field Sense Antisense
siRNA chemical modification 2-Fluoro* Cholesterol 2-Fluoro* Cholesterol
siRNA modification types 2 2
Overall number of modifications 6 13
Position of modifications 2,5,11,15,24 * 26 3,6,18,24,25,26,27,28 * 1,2,23,25,26
Modification on sugar or base or phosphate Sugar; Phosphate Sugar; Phosphate
SMILES
(Click to view structure & nomenclature) 		OCC1OCC(F)C1O
CC(C)CCCC(C)C1CCC2C3CC=C4CC(O)CCC4(C)C3CCC12C
OCC1OCC(F)C1O
CC(C)CCCC(C)C1CCC2C3CC=C4CC(O)CCC4(C)C3CCC12C
siRNA Sequence GUCAUCACACUGAAUACCAAUGCUGGA CACAGUAGUGUGACUUAUGGUUACGACCU
siRNA length base-pair 27 29
siRNA name in paper Toc-siRNA Toc-siRNA
Biological activity NA
Experiment used to check activity Real-time PCR
Melting temperature (oC) NA
Target gene Apolipoprotein B gene
siRNA concentration 10 nM
Cell or Organism used Hepa1-6 cells
Transfection method Lipofectamine RNAiMAX (Invitrogen)
Duration after transfection 24 Hours
Article title Efficient in vivo delivery of siRNA to the liver by conjugation of alpha-tocopherol
Reference18362929