SM3406 Chemically modified siRNA information

Field Sense Antisense
siRNA chemical modification 2-Fluoro* Cholesterol 2-Fluoro* Cholesterol
siRNA modification types 2 2
Overall number of modifications 11 11
Position of modifications 4,5,19,20 * 1,2,3,17,18,19,20 1,2,3,4,5 * 4,5,6,18,19,20
Modification on sugar or base or phosphate Sugar; Phosphate Sugar; Phosphate
SMILES
(Click to view structure & nomenclature) 		OCC1OCC(F)C1O
CC(C)CCCC(C)C1CCC2C3CC=C4CC(O)CCC4(C)C3CCC12C
OCC1OCC(F)C1O
CC(C)CCCC(C)C1CCC2C3CC=C4CC(O)CCC4(C)C3CCC12C
siRNA Sequence CGAUGUGUCUAUUGAAGAUUC CGGCUACACAGAUAACUUCUA
siRNA length base-pair 21 21
siRNA name in paper R1 R1
Biological activity NA
Experiment used to check activity Northern Blot
Melting temperature (oC) NA
Target gene Human SOD1 gene
siRNA concentration 20 nM
Cell or Organism used HEK293 cells
Transfection method Lipofectamine 2000
Duration after transfection 25 Hours
Article title Therapeutic RNAi targeting PCSK9 acutely lowers plasma cholesterol in rodents and LDL cholesterol in nonhuman primates
Reference18367449