SM3408 Chemically modified siRNA information

Field Sense Antisense
siRNA chemical modification 2-Fluoro* Cholesterol 2-Fluoro* Cholesterol
siRNA modification types 2 2
Overall number of modifications 10 3
Position of modifications 2,3,4,9,10,11,13,14,15 * 20 7,14 * 1
Modification on sugar or base or phosphate Sugar; Phosphate Sugar; Phosphate
SMILES
(Click to view structure & nomenclature)
OCC1OCC(F)C1O
CC(C)CCCC(C)C1CCC2C3CC=C4CC(O)CCC4(C)C3CCC12C


OCC1OCC(F)C1O
CC(C)CCCC(C)C1CCC2C3CC=C4CC(O)CCC4(C)C3CCC12C


siRNA Sequence GCCUGGAGUUUAUUCGGAATT TTCGGACCUCAAAUAAGCCUU
siRNA length base-pair 21 21
siRNA name in paper NA NA
Biological activity NA
Experiment used to check activity Real-time PCR
Melting temperature (oC) NA
Target gene PCSK9 gene
siRNA concentration NA
Cell or Organism used HepG2 cells
Transfection method Lipofectamine 2000
Duration after transfection 24 Hours
Article title Therapeutic RNAi targeting PCSK9 acutely lowers plasma cholesterol in rodents and LDL cholesterol in nonhuman primates
Reference18695239