Field | Sense | Antisense |
siRNA chemical modification | 2-O-Methyl | 2-O-Methyl* Cholesterol |
siRNA modification types | 1 | 2 |
Overall number of modifications | 2 | 3 |
Position of modifications | 20,21 | 1,2 * 1 |
Modification on sugar or base or phosphate | Sugar | Sugar; Phosphate |
SMILES (Click to view structure & nomenclature) |
COC1C(O)OC(CO)C1O |
COC1C(O)OC(CO)C1O CC(C)CCCC(C)C1CCC2C3CC=C4CC(O)CCC4(C)C3CCC12C |
siRNA Sequence | CUGAGUUUAAAAGGCACCCTT | TTGACUCAAAUUUUCCGUGGG |
siRNA length base-pair | 21 | 21 |
siRNA name in paper | Sirna-027 | Sirna-027 |
Biological activity | IC-50 = 0.05 nM for target mRNA | |
Experiment used to check activity | Real-time PCR | |
Melting temperature (oC) | NA | |
Target gene | VEGFR1 gene | |
siRNA concentration | 125 nM | |
Cell or Organism used | HeLa S3 cells | |
Transfection method | OptiMEM (Invitrogen) | |
Duration after transfection | 4 Hours | |
Article title | Suppression of ocular neovascularization with siRNA targeting VEGF receptor 1 |
|
Reference | 16195704 |