Field | Sense | Antisense |
siRNA chemical modification | 0 | 2-O-Methyl* Phosphodiester |
siRNA modification types | 0 | 2 |
Overall number of modifications | 0 | 11 |
Position of modifications | 0 | 6,12,15,18,19,20 * 6,12,15,18,19 |
Modification on sugar or base or phosphate | 0 | Sugar |
SMILES (Click to view structure & nomenclature) |
- |
COC1C(O)OC(CO)C1O OCC1OCC(O)C1OP(O)([O-])=O |
siRNA Sequence | CAUUGGUGAAGGAUCCAGGA | UCCUGGAUCCUUCACCAAUG |
siRNA length base-pair | 20 | 20 |
siRNA name in paper | 7 | 6 |
Biological activity | IC-50 = 22.859 nM for target mRNA | |
Experiment used to check activity | Real-time PCR | |
Melting temperature (oC) | NA | |
Target gene | Human PTEN gene | |
siRNA concentration | 200 nM | |
Cell or Organism used | MH-S cells | |
Transfection method | Lipofectin (Invitrogen) | |
Duration after transfection | 16 Hours | |
Article title | POSITIONALLY MODIFIED siRNA CONSTRUCTS |
|
Reference | US8394947, EP1765074B1 |