SM3413 Chemically modified siRNA information

Field Sense Antisense
siRNA chemical modification 0 2-O-Methyl* Phosphodiester
siRNA modification types 0 2
Overall number of modifications 0 13
Position of modifications 0 6,7,10,11,18,19,20 * 6,7,10,11,18,19
Modification on sugar or base or phosphate 0 Sugar
SMILES
(Click to view structure & nomenclature)
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COC1C(O)OC(CO)C1O
OCC1OCC(O)C1OP(O)([O-])=O


siRNA Sequence AGAAGACACCUUCUGAGUAU AUACUCAGAAGGUGUCUUCU
siRNA length base-pair 20 20
siRNA name in paper 9 8
Biological activity IC-50 = 75.517 nM for target mRNA
Experiment used to check activity Real-time PCR
Melting temperature (oC) NA
Target gene eIF4E gene
siRNA concentration 200 nM
Cell or Organism used MH-S cells
Transfection method Lipofectin (Invitrogen)
Duration after transfection 16 Hours
Article title POSITIONALLY MODIFIED siRNA CONSTRUCTS
Reference US8394947, EP1765074B1