SM3415 Chemically modified siRNA information

Field Sense Antisense
siRNA chemical modification 0 2-O-Methyl* Phosphodiester
siRNA modification types 0 2
Overall number of modifications 0 13
Position of modifications 0 6,7,10,11,17,18,19 * 6,7,10,11,17,18
Modification on sugar or base or phosphate 0 Sugar
SMILES
(Click to view structure & nomenclature)
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COC1C(O)OC(CO)C1O
OCC1OCC(O)C1OP(O)([O-])=O


siRNA Sequence GGAGAUCAACAUUUUCAAA UUUGAAAAUGUUGAUCUCC
siRNA length base-pair 19 19
siRNA name in paper 11 10
Biological activity IC-50 = 55.386 nM for target mRNA
Experiment used to check activity Real-time PCR
Melting temperature (oC) NA
Target gene Survivin gene
siRNA concentration 200 nM
Cell or Organism used HeLa cells
Transfection method Lipofectin (Invitrogen)
Duration after transfection 16 Hours
Article title POSITIONALLY MODIFIED siRNA CONSTRUCTS
Reference US8394947, EP1765074B1