| Field | Sense | Antisense |
| siRNA chemical modification | 2-O-Methyl* Phosphodiester | 2-O-Methyl* Phosphodiester |
| siRNA modification types | 2 | 2 |
| Overall number of modifications | 36 | 13 |
| Position of modifications | 2,3,4,5,6,7,8,9,10,11,12,13,14,15,16,17,18,19 * 2,3,4,5,6,7,8,9,10,11,12,13,14,15,16,17,18,19 | 6,7,10,11,18,19,20 * 6,7,10,11,18,19 |
| Modification on sugar or base or phosphate | Sugar | Sugar |
| SMILES (Click to view structure & nomenclature) |
COC1C(O)OC(CO)C1O OP(O)(O)=O |
COC1C(O)OC(CO)C1O OCC1OCC(O)C1OP(O)([O-])=O |
| siRNA Sequence | AGAAGACACCUUCUGAGUAU | AUACUCAGAAGGUGUCUUCU |
| siRNA length base-pair | 20 | 20 |
| siRNA name in paper | 9 | 8 |
| Biological activity | IC-50 = 261 nM for target mRNA | |
| Experiment used to check activity | Real-time PCR | |
| Melting temperature (oC) | NA | |
| Target gene | eIF4E gene | |
| siRNA concentration | 200 nM | |
| Cell or Organism used | MH-S cells | |
| Transfection method | Lipofectin (Invitrogen) | |
| Duration after transfection | 16 Hours | |
| Article title | POSITIONALLY MODIFIED siRNA CONSTRUCTS |
|
| Reference | US8394947, EP1765074B1 | |