SM3425 Chemically modified siRNA information

Field Sense Antisense
siRNA chemical modification 0 Locked nucleic acid* Phosphodiester
siRNA modification types 0 2
Overall number of modifications 0 6
Position of modifications 0 3,8,17 * 3,8,17
Modification on sugar or base or phosphate 0 Sugar
SMILES
(Click to view structure & nomenclature)
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OCC12COC(CO1)C2O
OCC1OCC(O)C1OP(O)([O-])=O


siRNA Sequence AAGUAAGGACCAGAGACAAA UUUGUCUCUGGUCCUUACUU
siRNA length base-pair 20 20
siRNA name in paper 13 12
Biological activity IC-50 = 809 nM for target mRNA
Experiment used to check activity Real-time PCR
Melting temperature (oC) NA
Target gene Human PTEN gene
siRNA concentration 200 nM
Cell or Organism used HeLa cells
Transfection method Lipofectin (Invitrogen)
Duration after transfection 16 Hours
Article title POSITIONALLY MODIFIED siRNA CONSTRUCTS
Reference US8394947, EP1765074B1