| Field | Sense | Antisense |
| siRNA chemical modification | 0 | Locked nucleic acid* Phosphodiester |
| siRNA modification types | 0 | 2 |
| Overall number of modifications | 0 | 12 |
| Position of modifications | 0 | 3,4,10,11,17,18 * 3,4,10,11,17,18 |
| Modification on sugar or base or phosphate | 0 | Sugar |
| SMILES (Click to view structure & nomenclature) |
- |
OCC12COC(CO1)C2O OCC1OCC(O)C1OP(O)([O-])=O |
| siRNA Sequence | AAGUAAGGACCAGAGACAAA | UUUGUCUCUGGUCCUUACUU |
| siRNA length base-pair | 20 | 20 |
| siRNA name in paper | 13 | 12 |
| Biological activity | IC-50 = 1570 nM for target mRNA | |
| Experiment used to check activity | Real-time PCR | |
| Melting temperature (oC) | NA | |
| Target gene | Human PTEN gene | |
| siRNA concentration | 200 nM | |
| Cell or Organism used | HeLa cells | |
| Transfection method | Lipofectin (Invitrogen) | |
| Duration after transfection | 16 Hours | |
| Article title | POSITIONALLY MODIFIED siRNA CONSTRUCTS |
|
| Reference | US8394947, EP1765074B1 | |